Children Vaccines

The manufacturing processes for vaccines have also come a long way, from using heat-inactivated cells to using recombinant DNA technology-driven antigen production. For example, inactivated polio vaccine as developed by Salk has been changed and improved and is currently being produced in a Vero cell line. Additionally, there are a few other examples of expression systems that are specifically developed for use in vaccine production. These include the PER.C6 cell line, the associated AdVac/Virosome technology (Crucell/DSM), and the avian-derived cell lines from Vivalis and ProBioGen. Another expression technology with potential benefits is the Pfenex Expression Technology from Dow, which has been used to generate high levels of vaccine antigens.

Vaccine production has several stages. Process of vaccine manufacture has the following steps:

• Inactivation – This involves making of the antigen preparation

• Purification – The isolated antigen is purified

• Formulation – The purified antigen is combined with adjuvants, stabilizers and      preservatives to form the final vaccine preparation.